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Mutant Search Help
Contents
Basic Mutant Search
How to use Advanced Search
About Search Results

Basic Mutant Search [ Top | Back ]
You can search Mutants with the following options:
  • Mutant FGSC IDs


  • A wildcard search using * can also be performed. Type the part of the name you know and use an asterisk (*) for parts you don't know. For e.g. 297* will give the search results for all the mutants found on FGSC plate 297.

    How to use Advanced Search [ Top | Back ]
    This section describes how to search for mutants using the Advanced Search page.
      1. Select the assay properties to search the mutants from the options below.



    For details on different types of assay type/values click here.
      2. Optional: Specify the search results output/display options. For more details on output option click here.
      3. Click the Search button to start the search.

    Output Options [ Top | Back ]

      You can specify following options to display on the search results page:
     
      Number of records per page
      You can select to display 20, 40, 60, 80 or 100 result items on a single page. More results per page will take longer to load.
      Show columns
      You can select the columns that you want to be displayed on the results page. For details about the columns click here. The default columns can be seen checked in the above image.
      Sort by
      You can select the column based on which you want to pre-sort your search results. The default results will be sorted by Mutant Id.


    About Search Results [ Top | Back ]
      This section describes the use of search results.
     

    Description of Table Columns

    Following are the details of the columns in the search results table:


    • Mutant ID: Shows the Mutant ID for the mutants found for the search. The Mutant ID are generated in PhenoDB at NCSU.

    • FGSC ID: Shows the Fungal Genetics Stock Center ID. The mutants can be ordered from using this ID from http://www.fgsc.net/.

    • Growth Rate: Radial growth of monoconidial cultures was scored on complete medium containing Hygromycin B (200mg/L) in 24-well plates. Growth was scored after 7 days of growth at 28°C. The rate of growth is compared to the average rate of the transformed lines instead of the parental strain (70-15), which is unable to grow in the presence of Hygromycin.

      Scoring
      Very slow mycelium fills 1/4 of well.
      Slow mycelium fills 1/2-3/4 of well.
      Normal mycelium fills most of the well (most strains fit this category).
      Fast mycelium fully fills the well.


    • Conidiation: Defects in sporulation are scored on oatmeal agar in 24-well plates after 7 days of growth at 25°C under constant fluorescent illumination.
      Scoring
      No sporulation no conidia are apparent.
      Low Less pigmentation than 70-15. May be normal mycelial growth with little pigmentation, although not white as in albino mutants.
      Normal Wild-type 70-15 growth with comparable pigmentation (in the case of pigmentation mutants, the intensity of the pigmentation as compared with mycelial growth)
      High Generally greater mycelial growth and greater intensity of pigmentation as compared to wild-type 70-15


    • Pigmentation: Defects in the melanin biosynthetic pathway produce pigment mutants called albino, buf or rosy. Pigment color was initially scored by the pigmentation of cultures grown on oatmeal agar 24-well plates after 7 days of growth at 25°C under lights:
      Scoring
      Gray Pigmentation like wild-type strain 70-15
      Albino white pigmentation
      Buff buff/ tan pigmentation
      Rosy reddish pigmentation


    • Auxotrophy:Strains were screened for ability to grow on minimal medium and complete medium in parallel. Assays were done in 24-well plates and scored after 10 days of growth at 28°C. Strains that failed to grow or exhibitied greatly reduced growth on miminal medium compared to strain 70-15, while able to grow on complete medium, indicates a putative nutritional defect. Additional tests to identify the specific nutritional defect were performed. Tests first involved supplementing media with groups of related compounds to place mutants into a category (vitamin, nuclei acids or amino acids). Next mutants were supplemented with individual compounds within the identified category to determine the specific defect.

    • Pathogenicity:
      Scoring
      0 non-pathogenic, no lesions on leaves
      1 reduced pathogenicity compared to 70-15. Fewer lesions or smaller lesions and limited or no effect on plant stature.
      2 lesion size and numbers equivalent to wild-type strain 70-15.
      3 hypervirulent, devastation of plants - greater than wild-type 70-15